Neutral Red staining..Help!!

[Darren D. Browning]

Hey there,

	I used to use both stains but never simultaneously...neutral red being
a pH sensitive vital dye works with living cells but hoechst, as far as I know
can't get into living cells.  You might try photographing the neutral red cells
then fixing them for hoechst afterwards for another shot.  Alternatively, a 
lysosomal enzyme marker antibody with a fitc or rhodamine secondary might work
for a single shot with dual fluor probes.

hope it helps.
Darren