Neutral Red staining..Help!!
Darren D. Browning
dbrownin at scripps.edu
Sat Mar 30 16:53:05 EST 1996
I used to use both stains but never simultaneously...neutral red being
a pH sensitive vital dye works with living cells but hoechst, as far as I know
can't get into living cells. You might try photographing the neutral red cells
then fixing them for hoechst afterwards for another shot. Alternatively, a
lysosomal enzyme marker antibody with a fitc or rhodamine secondary might work
for a single shot with dual fluor probes.
hope it helps.
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