How to prevent collagen from peeling off glass slides?
tvink at xs4all.nl
Sat May 4 14:10:02 EST 1996
dcwong at unixg.ubc.ca (Donald Chun Kit Wong) wrote:
>We have been trying to use the Nunc Tissue Culture Chamber Slides to
>culture endothelial cells. We routinely coat plastic culture plates with
>fibronectin or collagen and have great success in culturing cells.
>However, we've had very little success with these glass slides from Nunc.
>We've tried coating these glass slides with collagen alone, fibronectin
>alone, or collagen with fibronectin on top. We usually see large areas of
>especially collagen peeling off and curling up into string-like
>structures, with the endothelial cells still attached to them. So, we get
>nothing left on the slide. Nunc said these slides are untreated, so may be
>charged. Does anyone have any suggestions on how one prevents the collagen
>from peeling off glass slides? Thanks!
For endothelial cells we pretreat the glass coverslips with
glutaraldehyde wash, incubate with gelatin and fix this layer again
with glutaraldehyde. For an exact protocol send me an e-mail.
Department of Haematology
University Hospital Utrecht
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