MTT Positivity: Can Contamination show MTT+

[Rees Jean-Francois]

MTT is a redox sensitive indicator so you should also check wether any
reducing compound could be present in your medium. We've observed a steong
reaction between vit E and MTT

Good luck

Jean-Francois

In article (Dans l'article) <53gs6l$a08 at swsu65.swmed.edu>, "Mariappan,
Muthu Marthanda Rajan" <mariappan at utsw.swmed.edu> wrote (écrivait) :

> I am a Tissue Engineering Researcher. I have been growing some cells in 
> a polymer scaffold. As controls, I had been using plain plymer scaffolds 
> without Cells.
>         To quantitate the cell growth, I tried using the MTT assay.
> But the coltrols show a very strong reaction. I am yet very unclear 
> whether a false +ve MTT can be because of the Matrix scaffold itself or 
> whether I am dealing with something else.
>         I was wondering whether Contaminations can cause a +ve MTT., Of
course 
> I have taken precautions like Ethlylene Oxide sterilisation etc.,... but 
> still, is it possible to get a +ve MTT with bacterial or fungal 
> contaminations.
> _rajan
> mariappan at utsw.swmed.edu