MTT Positivity: Can Contamination show MTT+
Reesjf at bani.ucl.ac.be
Tue Nov 5 05:58:25 EST 1996
MTT is a redox sensitive indicator so you should also check wether any
reducing compound could be present in your medium. We've observed a steong
reaction between vit E and MTT
In article (Dans l'article) <53gs6l$a08 at swsu65.swmed.edu>, "Mariappan,
Muthu Marthanda Rajan" <mariappan at utsw.swmed.edu> wrote (écrivait) :
> I am a Tissue Engineering Researcher. I have been growing some cells in
> a polymer scaffold. As controls, I had been using plain plymer scaffolds
> without Cells.
> To quantitate the cell growth, I tried using the MTT assay.
> But the coltrols show a very strong reaction. I am yet very unclear
> whether a false +ve MTT can be because of the Matrix scaffold itself or
> whether I am dealing with something else.
> I was wondering whether Contaminations can cause a +ve MTT., Of
> I have taken precautions like Ethlylene Oxide sterilisation etc.,... but
> still, is it possible to get a +ve MTT with bacterial or fungal
> mariappan at utsw.swmed.edu
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