protease inhibitors

Limin Zheng lxzst at vms.cis.pitt.edu
Sat Nov 9 12:24:56 EST 1996


Could anyone tell me what kind of protease inhibitor I should use if I just
want them to stay in the medium but not getting into the cells? Plus, how
will it affect the cells cultured in conditioned medium with these inhibitors?
If I just need to get secreted proteins from medium, should protease be a
concern?
Thank you very much for your help.

Limin
Limin Zheng
Biological Sciences Dept.
University of Pittsburgh
Pittsburgh, PA 15260




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