dissociation question

[John Edstrom]

What is a good enzyme to use to remove extracellular matrix in DRG?  I
have tried many combinations of trypsin, collagenase and dispase on
from DRG and I can't get the glia clinging to the somata to let go. 

Any suggestions?

je


---
 John Edstrom | edstrom @ slugo.hmsc.orst.edu

 http://www.hmsc.orst.edu/~edstrom
 "Lurker" at BioMOO (bioinfo.weizmann.ac.il:8888)

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