dissociation question

John Edstrom edstrom at Poopsie.hmsc.orst.edu
Thu Nov 14 12:43:12 EST 1996

What is a good enzyme to use to remove extracellular matrix in DRG?  I
have tried many combinations of trypsin, collagenase and dispase on
from DRG and I can't get the glia clinging to the somata to let go. 

Any suggestions?


 John Edstrom | edstrom @ slugo.hmsc.orst.edu

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