apoptosis detection

[Richard A. Lockshin]

I will try to get a comment up on our web page shortly.  We'd be glad
to post any suggestions there as well
(http://rdz.stjohns.edu/~lockshin/index.html).
Li-Hsien Lin <lilin at blue.weeg.uiowa.edu> wrote:

>	I am having problem getting Apoptosis Detetion Kit that I
>purchased to work nicely. This is a TUNEL assay with fluorescein-dUTP. My
>tissue is 4% paraformaldehyde fixed (16h postfix) 20 um rat brain frozen
>sections. They are treated with Triton X-100 and Proteinase K before the
>TUNEL assay. On the postive control (DNase I treated), I only got the
>cells at the edges to show fluoresent staining. I will appreciate anyone who
>can give me suggestions about this problem. Thanks a lot. 


Richard A. Lockshin
(lockshin at mindspring.com;lockshin at sjumusic.stjohns.edu)