plasma membrane-selective permeabilization?

Dr E. Buxbaum EB15 at le.ac.uk
Tue Nov 26 10:39:27 EST 1996


gst3 at york.ac.uk wrote:
>Hi, 
>I'd be very grateful if anybody could point me in the direction of techniques  to 
>specifically permeabilize plasma membranes in cultured mammalian cells whilst 
>leaving as much else  (mito, lysosomes etc) unaffected.

The detergent digitonin will do exactly that. It binds preferentialy to 
membranes containing cholesterol, i.e. the cell membrane.





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