plasma membrane-selective permeabilization?
Dr E. Buxbaum
EB15 at le.ac.uk
Tue Nov 26 10:39:27 EST 1996
gst3 at york.ac.uk wrote:
>I'd be very grateful if anybody could point me in the direction of techniques to
>specifically permeabilize plasma membranes in cultured mammalian cells whilst
>leaving as much else (mito, lysosomes etc) unaffected.
The detergent digitonin will do exactly that. It binds preferentialy to
membranes containing cholesterol, i.e. the cell membrane.
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