Trouble with CaPO4 transfections
janeh at ihug.co.nz
Tue Oct 1 15:00:23 EST 1996
In article , says...
>Helge Steen wrote:
>>We've been using the CaPO4 method to transfect mammalian cells
>>3T3) successfully for years.
>>After a recent lab move we are now having problems because the
>>transfection efficiency is gradually decreasing. A recent test
>>X-gal staining revealed that the transfection efficiency is
>>This is less than 1/10th the efficiency we had before.
>>The precipitate we see is comparable to that we saw before the
>>neither of our assays (luciferase & beta-gal) have changed.
>If luciferase assay has not changed, perhaps you should try a
>of X-gal. Your transfection efficiency has probably not
>kevans at lifetech.com
we had exactly the same after moving to new labs. Turned out to
be the water quality, believe it or not.
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