cell death in plates

tonetti tonetti at ZMK.unibe.ch
Sun Oct 27 11:11:48 EST 1996


thomas.kreuzer at kfunigraz.ac.at (Thomas Kreuzer) writes:
> Hello fellow netters,
> 
> I have been faced with a problem recently , for which I can find no solution 
> altough it may be trivial.
> I seed HeLa - cells on standard 6-well cell culture plates (Costar) in 1 ml
> DMEM plus 5 % FCS , let them attach to the bottom overnight and then change 
> the medium according to the tests I want to perform afterwards. I have noticed 
> for some time that the cells in certain wells show a different behaviour than 
> the others. For example: If I number the wells from left to right 1 to 3 in 
> the upper row and 4 to 6 in the lower , the cells in number 4 are always more 
> difficult to get off the bottom of the well with trypsin. First I thought it 
> might be a handling problem during the tests , but last time I decided to let 
> the cells grow for one additional day on the plate before the test , so I did 
> not change the medium after the first night , but left it for further 24 
hours.
> When I looked through the microscope afterwards , I noticed that the cells in 
> wells 1,2,3 and 5 had grown well , while in number 4 and 6 most of the cells 
> were dead , altough an equal amount of cells had been attached to the bottom 
> after the first night in all wells. The conditions were the same for all 6 
> wells.  
> So my question is: has anybody noticed a similar effect or has some 
> suggestions why this might happen ?
> 
> Thanks in advance,
> Tom
> -----------------------------------------------
> Tom Kreuzer
> Institute for Biochemistry,K-F-University Graz,Halbaerthgasse 5,A-8010 
Graz,Austria
> thomas.kreuzer at kfunigraz.ac.at
> 




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