cell death in plates

[David Rusnak]

John Brennand wrote:
> 
> tom
> 
>cut/snip


> or it could just be a duff batch of plates - try an alternative
> supplier
> 

I hear this excuse a lot and I think it's overused.  I know someone who 
claimed that the outside wells of the plate don't get "tissue culture 
treated as well as the inside wells" and that's why her outside wells 
never grew as well as the inside wells.  

Don't change suppliers without adding more than 1 ml/well and making sure 
your incubator is well humidified.  You might be wasting a lot of good 
plates!! Evaporation is the most likely culprit.

Later,

Rusnak