Need help with reducing western background...

Darren Tyson tysondr at sluvca.slu.edu
Tue Sep 3 17:06:01 EST 1996


Dr. Jim Rutka wrote:
> 
> Any suggestions on reducing background on western detections (HRPO -
> Chemiluminescence on PVDF membrane)?
> 
> --
> Dr. James T. Rutka                             rutka at sickkids.on.ca
> Assistant Professor, Neurosurgery              Phone: (416)813-6425
> The Hospital for Sick Children                 Fax:   (416)813-4975
> University of Toronto, CANADA


What are your blocking conditions?  What are your primary and secondary
antibodies?  Is the background due to primary or secondary Ab?
-- 

Darren Tyson, Grad Student Cell and Molecular Biology
tysondr at sluvca.slu.edu or darren at primary.net
http://scimart.com/darren/



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