Need help with reducing western background...
tysondr at sluvca.slu.edu
Tue Sep 3 17:06:01 EST 1996
Dr. Jim Rutka wrote:
> Any suggestions on reducing background on western detections (HRPO -
> Chemiluminescence on PVDF membrane)?
> Dr. James T. Rutka rutka at sickkids.on.ca
> Assistant Professor, Neurosurgery Phone: (416)813-6425
> The Hospital for Sick Children Fax: (416)813-4975
> University of Toronto, CANADA
What are your blocking conditions? What are your primary and secondary
antibodies? Is the background due to primary or secondary Ab?
Darren Tyson, Grad Student Cell and Molecular Biology
tysondr at sluvca.slu.edu or darren at primary.net
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