Need help with reducing western background...

Darren Tyson tysondr at
Tue Sep 3 17:06:01 EST 1996

Dr. Jim Rutka wrote:
> Any suggestions on reducing background on western detections (HRPO -
> Chemiluminescence on PVDF membrane)?
> --
> Dr. James T. Rutka                             rutka at
> Assistant Professor, Neurosurgery              Phone: (416)813-6425
> The Hospital for Sick Children                 Fax:   (416)813-4975
> University of Toronto, CANADA

What are your blocking conditions?  What are your primary and secondary
antibodies?  Is the background due to primary or secondary Ab?

Darren Tyson, Grad Student Cell and Molecular Biology
tysondr at or darren at

More information about the Cellbiol mailing list