PBS - More or less ?!
Dpt. of Cell Morphology
Cell.Morphology at rz.ruhr-uni-bochum.de
Fri Sep 6 17:08:50 EST 1996
General account , VENETTA wrote:
> Can somebody advise on the composition and concentrations of a PBS buffer
> used for washing cells?!
Our Lab uses the following composition for fluorescently labelled cells
or western blots, which we consider to be the original Dulbecco one.
PBS nach Dulbecco
8,0 g/l NaCl
0,2 g/l KCl
0,1 g/l CaCl2
1,15 g/l Na2HPO4 * 2 H2O
0,2 g/l KH2PO4
0,1 g/l MgCl2 * 6 H2O
you may add 0,01 to 0,05% NaN3 for conservation purposes. You may also
make a 10-fold concentrated stock solution, but then adjust the pH after
I hope, it works
Ralf Breuker at the
Department of Cell Morphology
NDEF 05 / Ruhr University Bochum
44780 Bochum, FRG
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