Mammalian cell replica plating

Stephen M. Schwartz S.M.Schwartz at worldnet.att.net
Tue Sep 17 22:52:35 EST 1996



David Winterbourne <sghk100 at sghms.ac.uk> wrote in article
<51ln3r$1uo at mercury.hgmp.mrc.ac.uk>...
> In article <3239E363.3CA8 at berlex.com>, Mukund Nori says...
> >
> >Has anyone replica plated mammalian cells?  I would greatly appreciate a

> >protocol and source of reagents.
> 
> It is possible to prepare replicas on polyester filters, although this 
> probably only works for fibroblast-like cells. I have used this with CHO
and 
> it works well:
> 
> Raetz, C.R.H., Wermuth, M.M., McIntyre, T.M., Esko, J.D., and Wing, D.C. 
> Somatic cell cloning in polyester stacks. Proc.Natl.Acad.Sci.USA
79:3223-3227, 
> 1982.
> 
> Alternatively, you can seed cells at low density in microtest plates. As
the 
> number of cells per well follow a Poisson distribution, some wells will
have 
> no cells whereas others will have more than one. See the following
reference 
> for an example:
> 
> Winterbourne, D.J., Thomas, S., and Hermon-Taylor, J. Suppression of 
> anchorage-independent growth after gene transfection. Br.J.Cancer
68:251-258, 
> 1993.
> 
> -- 
> Dr. David Winterbourne
> Department of Surgery
> St George's Hospital Medical School, London SW17 0RE, England
> Tel: 0181-725-5581   Fax: 0181-725-3594
> 
> 




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