help on apoptosis: single cell marker

ecastro at eucmax.sim.ucm.es ecastro at eucmax.sim.ucm.es
Tue Sep 17 12:14:47 EST 1996


Hello,
  I am trying to enter in the field of apoptosis. I need a marker to determine
if individual cells in a culture are undergoing apoptosis or not. I have readed
papers using a technique based in the 3'-OH end extension of DNA fragments, but 
the detailed procedure is not there. The technique seems propietary of ONCOR,
Gaithersburg, MD. This company sells a kit to perform the reaction

I need:

a) The fax or e-mail of ONCOR (unknown to me and my colleagues here)
b) If possible, details in a non-kit approach to the same objetive

Thanks in advance to everybody

Enrique Castro
Dept. Bioquimica, UCM
ecastro at eucmax.sim.ucm.es




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