Kimjh at sorak.kaist.ac.kr
Sun Sep 22 12:17:26 EST 1996
I can't be quite sure about the information that you want but in present
Erythropoietin is not being synthesized by recombinant microorganisms.
Due to its heavily glycosylated structure bacteria nor yeast does
this glycoprotein. It is reported that even recombinant yeast couldn't
possibly produce Erythropoietin because of some secretion problem
to proper glycosyation.
Besides, there are quite a lot of reports on synthesis of Erythropoietin
animal cells (Chinese hamster ovary cells, Baby hamster kidney cells,
and insect cells (Sf9).
There's few reviews that I'd like to suggest as below;
Aggarwal, B., Jordan, U.G.,'Huamn cytokines(handbook for basic and
Blackwell scientific publication, 383-398
Koury, M., Maurice, C.B., 'The molecular machanism of Erythropoietin
Eur. J. Biochem., 210: 649-663
Masaaki, G. et al, ' Production of recombinant human Erythropoietin in
mammalian cells: host-cell
dependency of the biological activity of the
Bio/Technology, 6: 67-71
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