Cell freezing protocol

D. KIM dkim at nmsu.edu
Thu Sep 19 14:34:42 EST 1996


In article <v01510100ae6712885cd6@[205.149.179.73]> martin_cunningham at MOLDEV.COM (Martin Donal Cunningham, Ph.D.) writes:
>Would you be so kind as to post me directly with your recipe for freezing
>cells.  A debate has been raised, and I have been asked to put this request
>to this newsgroup in order that a consensus of opinion can be reached.
>Thank you for for taking the time.
>
>Martin Donal Cunningham, Ph.D.
>Research Scientist
>Molecular Devices Corporation
>1311 Orleans Dr.
>Sunnyvale, Calif. 94089
>Direct: 408-747-3524
>Fax: 408-747-3601


Hello:

What is the nature of the debate?
Mammalian cells?
I believe the consensus is to adjust the cell medium with DMSO (details 
vary) and then freeze slowly.  Slow freezing is accomplished in cheap or 
expensive ways.

Daniel Kim




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