separation of dead/alive T cell hybridoma cells
plaver at PO-Box.McGill.CA
Wed Sep 25 09:54:22 EST 1996
> bboverduin at ucdavis.edu (Bert Overduin) writes:
> I am looking for a method to separate alive and dead mouse suspension T
> cell hybridoma (3DO) cells other than fluorescence activated cell sorting.
> I am not very familiar with animal cell biological techniques, but guess
> there must be a way to accomplish this. Answers and or hints can dbe
> directly sent to me or posted on the newsgroup.
> Bert Overduin
> CEPRAP / UC Davis
> bboverduin at ucdavis.edu
Try spinning your cell suspension over a Ficoll-Hypaque gradient. I have successfully
done this to clean up both hybridoma cells as well as preparations of activated cells.
Cells harvested from the interface are usually 95-100% viable.
You will probably lose some cells in the process, so if cell numbers are crucial you may
want to try another method first.
Transplant Immunology Lab.
Royal Victoria Hospital/McGill University
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