How to remove biotin from proteins?
Salome.kantengwa at medecine.unige.ch
Tue Apr 1 08:28:21 EST 1997
Does somebody know a method to remove biotin from proteins on a
nitrocellulose membrane? My biotinylated proteins were
immunoprecipitated by antibodies of interest and biotin-streptavidin
complexes were revealed by ECL reagents. Now I would like to reuse
the membrane for Western Blot analysis with another antibody. Since I
have to detect the antigen by ECL, I need to remove biotin groups to
avoid interferences. Thank you for your help.
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