How to remove biotin from proteins?

Salom=?iso-8859-1?Q?=E9 Kantengwa Salome.kantengwa at medecine.unige.ch
Tue Apr 1 08:28:21 EST 1997


Hello,

Does somebody know a method to remove biotin from proteins on a 
nitrocellulose membrane? My biotinylated proteins were 
immunoprecipitated by antibodies of interest and biotin-streptavidin 
complexes were revealed by ECL reagents. Now I would like to reuse  
the membrane for Western Blot analysis with another antibody. Since I 
have to detect the antigen by ECL, I need to remove biotin groups to 
avoid interferences. Thank you for your help.

Salomé



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