Question : ES cell and Homogenization

Changjin Lee senovia at
Wed Apr 2 00:57:03 EST 1997

Hello to all.
This is Changjin Lee.
In using ES cell line to purify its membrane bound polysomal RNA,
I want to know how many times I should up and down Dounce(B) glass
ES cell aggregates doesn't seem to break easily.
Do you have any answer or advice?
This is applicable to embryonic body...

Best Regards

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