How to remove biotin from proteins?

Ziping Yang ziping at u.washington.edu
Mon Apr 7 17:57:26 EST 1997


Signal decays out but I think the biotin molecules still bind to the
proteins. My sense tells me we have to do something to remove biotin
before reprobing with another antibody, is that right?


In article <19970407005300.UAA04224 at ladder01.news.aol.com>, hhers at aol.com
(HHers) wrote:

> You don't have to remove the biotin!!!! The signal decays out. Therefore
> you can simply wait for a few days, then test your membrane by incubating
> with the substrate and exposing to film. You will find that there is no
> activity. Then  enzyme  you can incubate your membrane with your antibody.



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