Background problems with Ni-NTA HRP conjugate

padma reddy reddypvr at BU.EDU
Mon Apr 21 11:58:19 EST 1997


Hi. I am using the Ni_NTA HRP conjugate (from Quiagen) to screen cell 
lines for the expression of his tagged proteins. I am getting a lot of 
background in both 3% milk-PBS-Tween and 3% BSA in PBS-Tween when used 
as a blocking agent. The Ni-NTA HRP conjugate is  diluted in PBS-Tween 
without milk/BSA as recommended and I use the ECL kit from Amersham. Any 
suggestions?
Thanks
Padma



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