About the cell concentration

yamamoto, aki aki-yama at aix.or.jp
Wed Apr 23 10:31:39 EST 1997


Help me with an elementary problem,immediately, anyone!

 I want to concentrate and precipitate a small amount of caltured float
cell for the exchange of liquid medium by the centrifugation with an
eppendolf tube (1.5ml) .
 I have tried with 1,000 rpm, but this rate is too low to precipitate
cells. Tell me whoever knows the ordinary conditions, the rate and time.



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