About the cell concentration

Martin Offterdinger a8803349 at unet.univie.ac.at
Tue Apr 29 03:43:49 EST 1997


On Mon, 28 Apr 1997 18:24:06 +0100, rfischer at utmem1.utmem.edu (Bob
Fischer) wrote:

>In article <335E2C2C.3AC9 at mailgw.aix.or.jp>, aki-yama at aix.or.jp wrote:
>
>>  I have tried with 1,000 rpm, but this rate is too low to precipitate
>> cells. Tell me whoever knows the ordinary conditions, the rate and time.
>
>Approximately 300-500 X g for 5 minutes is sufficient for most mammalian
>cells; if you want them viable, I would not exceed 1000 x g for any
>mammalian or insect cell type. For bacterial/fungi/most protists, you
>12,000 x g for 1 minute will usually suffice.
>
>-- 
>Bob Fischer
>"Drink and be merry, for our time is short, and death last forever."
>                                                                        Amphis Fragment, ca 300 B.C.
I would not use 14000rpm in an eppendorf fuge, this will probably kill
your cells, 1000rpm on the other hand is to low to pellet the cells, I
normally use 3000-4000rpm, 5 min this will pellet the cells
efficiently but will not destroy their membrane.
Martin



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