Aspergillus nidulans protoplasts:help!!!!!

Roy O'Mahony IN5403 at
Wed Apr 30 05:52:44 EST 1997

Has anyone out there got a very efficient protocol for creating A 
nidulans protoplasts, (I need them for studies on cell volume regulation 
via image analysis)
I am especially interested in ways of seperating the protoplasts from the 
rest of the cells and mycelial debree.
and I would like to know of an efficient enzyme to use in creating fungal 



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