Anyone have a good protocol for Isolating Nuclei and Nuclear extracts??

Graham Dellaire dellaire at ODYSSEE.NET
Sat Aug 2 22:36:29 EST 1997


Hello all,

I am trying to isolate nuclei after transfection of murine fibroblasts
with CaPO4.  Does anyone have a reference or a good (easy, lowtech)
protocol for isolating nuclei and fractionating nucleoplasm from nuclear
matrix?

I want to look at where my vector goes... i.e. is it in the nucleoplasm
or associated with the matrix.

Thanks in advance for all your help,

Graham Dellaire
dellaire at odyssee.net

p.s. if possible please reply via e-mail



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