Looking for a postdoc postion in Cell biology
dongm at mayo.edu
dongm at mayo.edu
Mon Aug 4 17:29:31 EST 1997
I post this ad for a friend of mine who's looking for a postdoc position in
cell/molecular biology. Please reply directly to Dr. Liu via e-mail
(xuesp at cdm.imicams.ac.cn) if you are interested. Thanks.
OBJECTIVE: Postdoctoral Fellowship
NAME: Shiguang Liu, male, born in April 8, 1963, married with a child,
ADDRESS: Department of Cell Biology, Institute of Basic Medical
Sciences,Chinese Academy of Medical Sciences(CAMS), Peking Union Medical
College(PUMC), 5 Dong Dan San Tiao, Beijing, 100005, P. R. China.
Tel: (86)(10)65296976 Fax: (86)(10)65133604
Ph.D. Cell Biology, Institute of Basic Medical Sciences, CAMS & PUMC,
China. July, 1997.
M.D. Obstetrics & Gynecology, Henan Medical University, Zhengzhou, China.
B.M. Medicine, Henan Medical University, Zhengzou, China. 1983.
1994-present: Ph.D. student. Department of Cell Biology, CAMS & PUMC.
1. Study on correlation between cytoskeletal filaments and denuceation
---Examining the dynamic change of quantitative and spatial distribution of
cytoskeletal filaments (vimentin, tubulin and actin) in different
stages of cultured splenic erythroblasts infected with anemia-inducing
of Freind virus (FVA cell) using immunofluorecence technique and laser
2. Study on apoptosis in FVA cells.
---Observing the change of cytoskeletal filaments and morphology.
---Determining the intracellular calcium concentration.
---Detecting DNA cleavage with in situ nick translation and
3. Subtractive cloning of gene related to erythroid differentiation and
denucleation in FVA cells.
---Isolating and culturing the splenic erythroblasts.
---Extracting and purifying mRNAs in two different developmental stages.
---Constructing two cDNA libraries.
---Identifying cDNA fragments related to erythroid differentiation and
denucleation using subtractive hybridization and PCR. Confirming by using
Northern blot assay. Four differentially expressed cDNA fragments are
and sequenced. One of them has been submitted to GenBank (AA114369).
---Demonstrating the specific expression of the genes with in situ
---Subcloning the differentially expressed cDNA into pLXSN retrovirus
and transfecting it into MEL cells.
---Observing the cell phenotype alteration of MEL cells in growth rate,
division index, colony forming rate and expression of beta-globin.
4. Cloning and expression of IL-6 in BL21(DE3)/PET-28 system.
1991-1994: Laboratory of Human Reproduction & Genetics, Department of
Obstetrics & Gynecology, The First Affiliated Hospital, Henan Medical
---In vitro fertilization (IVF) and gamete intrafallopian transfer (GIFT).
---Cytogenetic examination and prenatal diagnosis.
---Study on infection of papillomaviruses and p53 point mutation in
---Study on gene therapy for beta-thalassemia: expression of retrovirally
transduced human beta-globin gene in fetal mouse liver cells.
1988-1991: MD student. Department of Obstetrics & Gynecology, Henan Medical
---Study on the diagnostic value of the zona-free hamster oocyte
assay in male infertility.
---Detecting DNA strand breaks in HeLa cells and 2BS cells induced by
Yuanhuacine (a main element of a Chinese herbal medicine used to induce
abortion) using in situ nick translation.
1983-1988: Obstetrician & Gynecologist. Department of Obstetrics &
Gynecologist, The First Affiliated Hospital, Henan Medical University.
Cell Biology: tissue culture, cell fusion, transfection,
and immunofluorecence technique, laser confocal scanning microscopy, in
nick translation, in situ hybridization.
Molecular Biology: DNA and RNA isolation, DNA recombination, gene
Southern blot, Northern blot and Western blot, RT-PCR, constructing and
screening cDNA library, nucleic acid sequencing, subtractive cloning,
differential display, extraction and purification of protein.
1. Shiguang Liu, et al. Apoptosis in erythroid progenitor cells. Acta
Anatomica Sinica 1996;27:376.
2. Shiguang Liu, et al. Isolation of cDNA fragments related to erythroid
differentiation and denucleation from murine splenic erythroblasts infected
with FVA by subtractive hybridization and PCR. (abstract). Acta Anatomica
3. Hongwei wang, Shiguang Liu, et al. Expression of retroviral transduced
human beta-globin gene in mouse fetal liver cells. Acta Academicae
4. S. Xue, H. Sun, Q. Du, J. Xin, S. Liu. Correlation between cytoskeletal
filaments and the two stages of denucleation process in mammalian
erythroblasts. 2nd International Malpighi Symposium, Rome, September, 1995.
5. Zuopeng Chang, Wen Tang, Shiguang Liu. Detection and analysis of gene
frequency of ABO blood group in 143 case of Tibetan nationality. Chinese J.
Eugenics and Heredity. 1995;3(1):46.
6. Shiguang Liu, et al. The diagnostic value of human sperm-hamster oocyte
penetration assay in male infertility. J. of. Henan Medical University.
7. Shiguang Liu, et al. Prenatal diagnosis of cytomegalavirus infection
PCR. Chinese J. of Eugenics and Heredity. 1995;3(6):26.
Boating Shao, Aijun Chen, Shiguang Liu, et al. Suppression of the
phenotype of Caski cell line by wild type p53 or Rb gene. Basic Medical
Sciences and Clinics. 1995; 15: 207
8. Shiguang Liu, et al. Analysis of human papillomaviruses and p53 point
mutation in cervical carcinoma of Chinese women. Tumor.1994; 14: 521.
9.Yingpu sun, Shiguang Liu, et al. Cytogenetic analysis in 81 married
with spontaneous abortions. Chinese J. of Eugenics and Heredity.
10. Shiguang Liu, et al. Detecting DNA strand breaks in HeLa cells and 2BS
cells induced by Yuanhuacine using in situ nick translation. Chinese
Traditional and Herbal Drugs. 1992;23:524.
Changjiang Dong, Yixun Wu, Shiguang Liu. Reversal of female sterilization
microsurgical techniques. Chinese J. of Microsurgery. 1988;11:73.
11. Shiguang Liu, et al. The study of BL(DE3)/PET-28 as an Expression
human IL-6. Acta Academicae Medicinae Sinica (accepted).
Provided upon provided
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