Calcium Crimson ?
icshin at bioneer.kaist.ac.kr
Thu Aug 14 03:30:58 EST 1997
I'm working with Calcium Crimson dye (Molecular Probe)
to monitor changes in calcium concentration in COS cells.
When I tried to observe the Calcium Crimson-loaded
cells with Confocal Laser Scanning Microscope (Bio-Rad),
I noticed that not all the cells are readily labeled with
Calcium Crimson, which is quite different from my
previous experiments with other calcium indicators
like Fluo-3 etc. Even worse, the few Calcium Crimson
labeled cells appeared to be non-viable by microscopic
Is Calcium Crimson is somewhat more toxic than
other fluorescent calcium indicators ?
Also I'd like to know the appropriate labeling period
and working concentration of Calcium Crimson.
Any of your kind help would be greatly appreciated,
Dept of Biochemistry
Hanyang Medical School
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