Hek 293, tsa 201, g418 and stable transfection

Gonzalo Ferreira gferreir at rush.edu
Mon Dec 1 13:43:45 EST 1997


Hi,

I am looking for any reference concerning the concentration of G418 that
must be used to select resistant clones of transfected Human Embryonic
Kidney cells (HEK 293 or tSA 201). 
I will also appreciate any help regarding selection of cells for stable
transfection of these cell lines.

Thanks for the information,


Gonzalo Ferreira
Dept. of Physiology and Molecular Biophysics.
Rush Univeristy.
1750 West Harrison St. jelke 1279
Chicago IL 60612



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