anti-p53 stain - help needed

Don vasiliev at cityline.ru
Fri Dec 19 14:02:43 EST 1997


Hello

does anybody have a decent protocol for anti-p53 staining? (cells, not
secs) I've come to methanol/acetone 1:1 15' 25C, then air dry and rehydr
w/PBS for fix. Block before 1st Ab (pAb421), 0.2%BSA/PBS for 45-60'
Still, non-spec background is much too high. I've tried to block w/NFDM
or caseine, but no go. Tween-20/PBS - practically no diff

Thanx

D




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