Looking for Apoptosis/Necrosis Assay

kai.kehe at medicus.fs.shuttle.de kai.kehe at medicus.fs.shuttle.de
Sun Feb 2 04:10:48 EST 1997


gilbride at bu.edu (Kevin Gilbride) wrote:


>> I'm working with an adherent cell line (keratinocytes). For screening
>> toxicological effects I'm looking for a good assay to measure the
>> percentage of apoptotic and necrotic cells after exposure.

>   Necrosis can be assayed by measuring the amount of lactate
>dehydrogenase released by the cells. See ? Warburton: Dev. or Dev.
>Biology. It's simple and cheap.

LDH-Efflux is a simple test. But I'm looking for a test to screen the
amount of necrotic and apoptotic cells simultanously.

 Sincerely,
 
 Kai Kehe

 >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>
 Institute of Pharmacology and Toxicology, Garching, Germany
 e-Mail: 	kai.kehe at fbw.baynet.de
	kai.kehe at medicus.fs.shuttle.de
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