Freezing Cell

Andrei Popov andrei.popov at bbsrc.ac.uk
Sun Feb 2 12:20:01 EST 1997


Rocky Ho wrote:
> 
> Dear collegues,
> 
> I am now going to freeze hepatocyte culture. I encounter two suggestion
> for the storage medium. One is Culture medium with DMSO and the other is
> FCS with 10% glycerol. Does anyone has idea on the benefit of using
> culture medium or FCS as storage medium?
> 
> Rocky Ho


For all cell types I ever worked with, the best was FCS + 7-10%
DMSO. Sometimes I used NCS instead of FCS. Then overnight at 70C
and transfer in LN for long-term storage. Glycerol is OK for E.coli or
S.cerevisiae.

Best wishes

Andrei



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