Freezing Cell

Andrei Popov andrei.popov at
Sun Feb 2 12:20:01 EST 1997

Rocky Ho wrote:
> Dear collegues,
> I am now going to freeze hepatocyte culture. I encounter two suggestion
> for the storage medium. One is Culture medium with DMSO and the other is
> FCS with 10% glycerol. Does anyone has idea on the benefit of using
> culture medium or FCS as storage medium?
> Rocky Ho

For all cell types I ever worked with, the best was FCS + 7-10%
DMSO. Sometimes I used NCS instead of FCS. Then overnight at 70C
and transfer in LN for long-term storage. Glycerol is OK for E.coli or

Best wishes


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