Transfection of B cells

Erik Kul| Hagen e.k.hagen at labmed.uio.no
Mon Feb 3 08:20:19 EST 1997


We're trying to transient transfect different B cell-lines (Ramos, Raji, 
U698) using electroporation. We have tried different voltage, 
capacitance, with and without DEAE-dextran etc. with no satisfactory 
results. Does anyone have any experience in successfully transfecting any 
one of these above mentioned cell lines, if so I would be very pleased if 
you could reply.

Thank you for your attention!

Malfrid Roe
LIIPAT, The national hospital of Norway. 




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