We're trying to transient transfect different B cell-lines (Ramos, Raji,
U698) using electroporation. We have tried different voltage,
capacitance, with and without DEAE-dextran etc. with no satisfactory
results. Does anyone have any experience in successfully transfecting any
one of these above mentioned cell lines, if so I would be very pleased if
you could reply.
Thank you for your attention!
LIIPAT, The national hospital of Norway.