Percolls-cell clumping

[Labo Longziekten]

In article <32EE2006.4C07 at bbsrc.ac.uk>, Colin Farquharson 
<Colin.Farquharson at bbsrc.ac.uk> writes:

>I am trying to separate chondrocytes into their different sizes using
>Percoll (Pharmacia) discontinuous gradients (1.04 - 1.08).
Things often works but often there is
>extensive clumping at the 1.06 band and this results in poor
>separation.

Did you ever try to add some DNAse to your cell suspension before you separate 
them? We do when we isolate cells from the lung. We used concentrations of 
0.01-0.05 mg/ml.

I hope this will help.

Frans J. van Overveld, PhD
Dept. Resp. Med.
Univ. Antwerp, Belgium