overveld at uia.ua.ac.be
Mon Feb 3 08:01:04 EST 1997
In article <32EE2006.4C07 at bbsrc.ac.uk>, Colin Farquharson
<Colin.Farquharson at bbsrc.ac.uk> writes:
>I am trying to separate chondrocytes into their different sizes using
>Percoll (Pharmacia) discontinuous gradients (1.04 - 1.08).
Things often works but often there is
>extensive clumping at the 1.06 band and this results in poor
Did you ever try to add some DNAse to your cell suspension before you separate
them? We do when we isolate cells from the lung. We used concentrations of
I hope this will help.
Frans J. van Overveld, PhD
Dept. Resp. Med.
Univ. Antwerp, Belgium
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