Percolls-cell clumping

Labo Longziekten overveld at
Mon Feb 3 08:01:04 EST 1997

In article <32EE2006.4C07 at>, Colin Farquharson 
<Colin.Farquharson at> writes:

>I am trying to separate chondrocytes into their different sizes using
>Percoll (Pharmacia) discontinuous gradients (1.04 - 1.08).
Things often works but often there is
>extensive clumping at the 1.06 band and this results in poor

Did you ever try to add some DNAse to your cell suspension before you separate 
them? We do when we isolate cells from the lung. We used concentrations of 
0.01-0.05 mg/ml.

I hope this will help.

Frans J. van Overveld, PhD
Dept. Resp. Med.
Univ. Antwerp, Belgium

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