Dr E. Buxbaum
EB15 at le.ac.uk
Tue Feb 4 12:40:41 EST 1997
delgadoi at PILOT.MSU.EDU ("Ivan J Delgado") wrote:
> A question regarding glycosylation of proteins and how that looks in
> a western.
Glycosylation leads to spread out, unsharp bands in SDS-PAGE (and
consequentially in Westerns), which have a higher molecular weight than
the unglycosylated protein.
A nice way to check for glycosylation is to stain the gel or the western
blot with Feulgen's reagent. Gives bright red bands, a very beautifull
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