glycosylation

Dr E. Buxbaum EB15 at le.ac.uk
Tue Feb 4 12:40:41 EST 1997


delgadoi at PILOT.MSU.EDU ("Ivan J Delgado") wrote:
>
> A question regarding glycosylation of proteins and how that looks in 
> a western.

Glycosylation leads to spread out, unsharp bands in SDS-PAGE (and 
consequentially in Westerns), which have a higher molecular weight than 
the unglycosylated protein. 

A nice way to check for glycosylation is to stain the gel or the western 
blot with Feulgen's reagent. Gives bright red bands, a very beautifull 
color.




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