I have been trying to use SLO to selectively introduce pores in the
plasma membrane of cells. I do this by incubating the cells with SLO in
an ice-water bath and then incubate the cells at 37 after washing the
unbound SLO. However I find that the ER compartment is not really
intact as I get a substantial amount of leakage. Can someone who has
this system going give me some tips.