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293 cells in serum-free medium?

Lloyd Graham graham at dbe.csiro.au
Tue Feb 18 00:19:08 EST 1997

Does anybody know if 293 cells (Human Embryonic Kidney) tolerate
serum-free or reduced serum culture medium, and for how long? I'm hoping
to purify a protein secreted from a stable 293 line, and the less serum I
can get away with, the easier it will be.

Many thanks,


Name:         Dr L.D. Graham
Organisation: CSIRO Division of Biomolecular Engineering
Location:     Sydney Laboratory
Address:      P.O. Box 184, North Ryde, NSW 2113, Australia
Phone:        (02) 9886 4803
Fax:          (02) 9886 4805
Email:        graham at dbe.csiro.au

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