ELISA against Quantitative Western Blot

Dr E. Buxbaum EB15 at le.ac.uk
Thu Feb 20 07:42:31 EST 1997


Rocky Ho <RockyHo at cuhk.edu.hk> wrote:
>Hi
>
>Commerical ELISA kit I encounted is always stated to be used to detect
>protein factors in the blood. If we want to quantify the amount of
>certain cellular protein, tissue protein extraction and Western blot
>must be carried out, and Chemiluminescence detection method then used to
>quantify the amount of protein unit for different tissue sample.

Rather than using Western blotting, I'd go for dot blotts. This not only 
saves time, but should also be less error prone (transfer efficiency) and 
also easier to quantify (defind spot shape). Of course, you have to 
verify by Western that only one antigen is reacting with your antibody.




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