293 cells in serum-free medium?

[Jarkko Kortesmaa]

In article <graham-1802971625090001 at tastiger.dbe.csiro.au>, graham at dbe.csiro.au (Lloyd Graham) wrote:
>Does anybody know if 293 cells (Human Embryonic Kidney) tolerate
>serum-free or reduced serum culture medium, and for how long? I'm hoping

I have used the insulin-transferrin-selen supplement (Sigma) to replace serum 
for 293 cells. The cells do not like it too much but survive at least two days 
in it. There is minimal or no cell growth, though. 

Good luck,. 

-------------------------------------------------------------------------
 Jarkko Kortesmaa                    Dept. of Matrix biology, MBB
 email: Jarkko.Kortesmaa at mbb.ki.se   Karolinska Institutet
                                     Stockholm, Sweden
-------------------------------------------------------------------------