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vital dye in the visible range

Kiran Bhadriraju bhad0001 at GOLD.TC.UMN.EDU
Tue Feb 25 10:09:09 EST 1997


I am in search of a dye to stain living cells which would absorb in the 
in the visible range.  I looking at hepatocytes through an inverted 
microscope (attached to an atomic force microscope, but that is 
irrelevent) and they don't show very well at all.  The cells are on a 
glass cover  slip stuck to a steel AFM specimen stub.    So my idea is to 
use a dye in conjunction with a white background for the coverslip. Even 
if the dye doesn't harm cells only for a few hours, and gives a slight 
increase in contrast, it would work fine.  Would coomassie blue work? (pl 
excuse if this is a repost, i am not sure if my last mail got through). 
Many thanks - Kiran

bhad0001 at gold.tc.umn.edu

Kiran Bhadriraju                                        campus mail Box-11, UMHC
Biomedical Engineering Institute
University of Minnesota, Twin Cities
Ph: (612) 626-6360 - (care Dr Hansen)

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