vital dye in the visible range

Christian Ammon hnok159 at mail.uni-wuerzburg.de
Wed Feb 26 14:03:22 EST 1997


Kiran Bhadriraju wrote:
> 
> Hi!
> 
> I am in search of a dye to stain living cells which would absorb in the
> in the visible range.  I looking at hepatocytes through an inverted
> microscope (attached to an atomic force microscope, but that is
> irrelevent) and they don't show very well at all.  The cells are on a
> glass cover  slip stuck to a steel AFM specimen stub.    So my idea is to
> use a dye in conjunction with a white background for the coverslip. 


I cant help you with the dye, but are you already using Nomarski 
Contrast?


christian ammon



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