Ok, here's the problem. I know there is probably nothing like "the" good
method but still I would like to figure out the following.
I am drifting into the direction of cell cycle regulation a little bit with
regard to my research topic at the moment. I have read some review articles
and some general articles about cell cycle synchronization. I know most of
the chemicals I can order and were they interfere. I have tried several of
them. However, what I realy miss as a detailed description of the possible
way to use the several methods,and more important, to check if I realy
reached the my goal. We do not have an easy acces to a FACS.
So can anybody give me some more detailed information with reagard to my
question (reviews, books?) and does anybody have some experience in
synchronizing CCL91.1 (3T3) cells?