How to separate vegf dimer into monomer?

Ziping Yang ziping at u.washington.edu
Fri Feb 28 11:33:35 EST 1997


Hi, everybody
I run vegf western and use B-mercaptoethanol (freshly added to lysis
buiffer at 280-400 mM) as a reducing reagent. Samples were boiled before
loading to gel. However,  VEGF dimer is always there and showed much
strong band than monomer did. Any suggestions are wellcomed.
ziping



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