Are small proteins underestimated on SDS-PAGE?

Lee, Ji Hyun newera at plaza.snu.ac.kr
Tue Jan 7 02:23:32 EST 1997


When much proteins of large molecular weight co-exist, do proteins of
small molecular weights tend to be underestimated on SDS-PAGE? In other
words, compared to their absolute contents their bands look light in
mixture of very much of other proteins but when only they are SDS-PAGEd
their bands look dark despite of the same contents.
Is it possible? What reference is helpful to me?
Any comments will be appreciated.

Thank you.



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