Small proteins are underestimated on SDS-PAGE?

Lee, Ji Hyun newera at
Tue Jan 7 07:40:04 EST 1997

When much proteins of large molecular weight co-exist, do proteins of

small molecular weights(6500 dalton) tend to be underestimated on
In other words, compared to their absolute contents their bands look
light in

mixture of very much of other proteins but when only they are SDS-PAGEd

their bands look dark despite of the same contents.

Is it possible? 

Any comments and any reference will be appreciated.

Thank you.

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