Looking for Apoptosis/Necrosis Assay

Kevin Gilbride gilbride at bu.edu
Fri Jan 17 13:37:58 EST 1997


In article <59ena1$jjg at narses.hrz.tu-chemnitz.de>, <none> (Kai Kehe) wrote:

> Hello,
> 
> I'm working with an adherent cell line (keratinocytes). For screening
> toxicological effects I'm looking for a good assay to measure the
> percentage of apoptotic and necrotic cells after exposure.
> 
> Sincerely,
> 
> Kai Kehe
> 
> Institute of Pharmacology and Toxicology, Garching, Germany
> e-Mail: kai.kehe at fbw.baynet.de


Hello Kai,
   Necrosis can be assayed by measuring the amount of lactate
dehydrogenase released by the cells. See ? Warburton: Dev. or Dev.
Biology. It's simple and cheap.

Good Luck, 

Kevin



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