transient transfection of polarized epithelial cells

darren cikaluk dcikaluk at anatomy.anat.med.ualberta.ca
Wed Jan 22 11:01:41 EST 1997


I guess? depends upon cell type I suppose?
I have stably transfected vero c1008, MDCK II and Caco-2 cells, and if I
wanted transients, I would have used almost exactly the same protocal.
What cell type are you trying to transfect? What pore density, diameter
and type of inserts?

Mike

mgarbutt at gpu.srv.ualberta.ca



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