Peroxidase problems

Wes Rose rose3 at JEFLIN.TJU.EDU
Fri Jan 24 09:50:44 EST 1997


I'm trying to use kpl's Histomark Black kit (peroxidase) for
immunostaining, but I'm getting high background staining (nuclear), even
in my controls.  After permiabilizing, I'm incubating O/N at 37=B0C in
0.3% H2O2, and blocking for 1 hr at 37=B0C with 3% BSA, prior to primary
Ab addition.  Between steps, I'm washing 4X.  Any suggestions would be
greatly appreciated.



More information about the Cellbiol mailing list