CLONING LONG RANGE PCR PRODUCTS/PFU treatment

Terrie-Anne Cock t.cock at garvan.unsw.edu.au
Mon Jan 27 23:34:00 EST 1997


Hello,

Has any one had success blunt end cloning Long Range PCR products. If you
have could you please email the conditions. We are trying to clone 2.0 to
7.0 kb fragments into pUC 18 SmaI site. The LR-PCR products are treated
with Pfu before cloning, has anyone had problems cloning Pfu treated PCR
products.

Any suggestions would be greatly appreciated,

Thanks

Terrie-Anne

___________________________________________________________________
Terrie-Anne Cock
Garvan Institute of Medical Research
384 Victoria St Darlinghurst                   Tel:   (02) 9295 8260
                                                           9295 8369
Sydney NSW 2010                                Fax:   (02) 9295 8241
Australia                       email: t.cock at garvan.unsw.edu.au
___________________________________________________________________






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