about RT-PCR primer designing

Zhigang Jin jinzg at HOTMAIL.COM
Wed Jun 4 11:17:31 EST 1997


Could any one tell me the important factors in choosing RT-PCR primers?
I try to detect mRNA level of PP 2A, PP 2B in human blood samples. But
these enzymes are heteropolymers. I don't know if I choose their
catalytic subunits can achieve the good results. I really wish some
experts can give some good advice. Thank in advance. Please mail to:
jinzg at hotmail.com.

Zhigang JIn



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