Homemade Chemiluminescent Protocols?

Eli Hestermann ehestermann at whoi.edu
Wed Jun 4 09:16:40 EST 1997

David Aldridge wrote:
> *Luminol 4mg/ml in DMSO         1ml
> *p-iodophenol 1mg/ml in DMSO    1ml
> 1M Tris.HCL pH 7.5              0.6ml
> H2O2 (30%)                      5 microL
> H2O                             7.5ml
> *Store at -20
> Mix this stuff up fresh a few minutes before use.
> Works for me!

I assume this is for HRP, does it work for AP, too?  If not, does
anybody have one for AP?

Eli Hestermann

Eli V. Hestermann
ehestermann at whoi.edu
Woods Hole Oceanographic Institution
Massachusetts Institute of Technology
"Vita brevis est, ars longa" - Seneca

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